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磷酸化蛋白激酶R重組兔單克隆抗體
  • 產品貨號：
    BN42102R
  • 中文名稱：
    磷酸化蛋白激酶R重組兔單克隆抗體
  • 英文名稱：
    Rabbit anti-Phospho-PKR (Thr446) Monoclonal antibody
  • 品牌：
    Biorigin

  • 貨號

    產品規格

    售價

    備注

  • BN42102R-50ul

    50ul

    ￥2020.00

    交叉反應：Human,Mouse,Rat 推薦應用：WB,IHC-P,IHC-F,ICC,IF

  • BN42102R-100ul

    100ul

    ￥3240.00

    交叉反應：Human,Mouse,Rat 推薦應用：WB,IHC-P,IHC-F,ICC,IF

產品描述

英文名稱Phospho-PKR (Thr446)
中文名稱磷酸化干擾素誘導的雙鏈RNA活化蛋白激酶重組兔單克隆抗體
別    名PKR(T446); PKR (phospho T446); p-PKR (phospho T446); PKR(Phospho-Thr446); double-stranded RNA-dependent Protein Kinase; interferon-induced, double-stranded RNA-activated protein kinase isoform a; protein kinase, interferon-inducible double stranded RNA dependent; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; protein kinase RNA-activated; interferon-inducible RNA-dependent protein kinase; EIF2AK2; EIF2AK1; MGC126524; PKR; PRKRv; E2AK2_HUMAN.  
產品類型磷酸化抗體 
研究領域腫瘤  免疫學  信號轉導  細胞凋亡  轉錄調節因子  激酶和磷酸酶  
抗體來源Rabbit
克隆類型Monoclonal
克 隆 號10A1
交叉反應Human, Mouse, Rat, 
產品應用WB=1:500-2000 IP=1:20-100 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量62kDa
細胞定位細胞核 細胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human PKR around the phosphorylation site of Thr446:KR(p-T)RS 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產品介紹PKR is an interferon-inducible serine/threonine specific protein kinase. It is widely expressed in eukaryotic organisms and activated by double stranded RNA. Activation of PKR by dsRNAs leads to autophosphorylation at multiple sites. Phosphorylation of Thr446 and Thr451 in the PKR activation loop is required in vivo and in vitro for high level kinase activity. PKR phosphorylates its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor 2 (EIF2 alpha), leading to the inhibition of protein synthesis. PKR is also involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, and also activates IKK and NFKB, thereby suppressing apoptosis. Recently, it has been reported that PKR also phosphorylates human p53 on serine 392. PKR might play a role in ER stress-induced apoptosis and in Alzheimer's disease. Alzheimer cases show prominent PKR activation in association with neuritic plaques and pyramidal neurons in the hippocampus and neocortex.

Function:
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. In adddition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity: phosphorylates CDK1 upon DNA damage. CDK1 phosphorylation triggers CDK1 polyubiquitination and subsequent proteolysis, thus leading to G2 arrest.

Subunit:
Homodimer. Interacts with STRBP (By similarity). Interacts with DNAJC3. Inhibited by direct interaction with viral proteins such as HCV E2, HCV NS5A and influenza A NS1. Activated by the interaction with HIV-1 Tat. Forms a complex with FANCA, FANCC, FANCG and HSP70.

Post-translational modifications:
Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 protein kinase domain.

SWISS:
Q52M43

Gene ID:
5610

Database links:

Entrez Gene: 5610 Human

Omim: 176871 Human

SwissProt: P19525 Human

Unigene: 131431 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications